Saturday, February 16, 2013

Mutation Types


Mutations are the source of new alleles in a species. Mutations can be beneficial, harmful, or have no effect. There are many types of mutations. The first type of mutation is a point mutation. A point mutation changes only a singe base pair. So if your DNA read ACTGTA a point mutation DNA would read ACTTTA, the highlighted T is where your point mutation occurred. Point mutations occur because random errors in DNA synthesis or random errors in the repair of damaged sites. Replacement substitutions are a form of point mutation that results in an amino acid change. On the other hand, point mutations that do not change the amino acid are called silent substitutions. Amino acids are coded from the three base pair codon, but some codons code for the same amino acid. For example if the codon TAC codes for the amino acid leucine and TAT also codes for leucine. The C to T point mutation would be a silent mutation since it codes for the same amino acid. The Wobble Hypothesis states that the third base pair in an anticodon can align in several ways to allow it to recognize more than one base in the codons of mRNA. A second form of mutation is Transversion. A transversion mutation occurs when a purine is substituted for a pyrimidine or vise versa. A purine in DNA is either adenine or guanine. A pyrimidine is cytosine, thymine, and in the case of RNA uracil. So if your DNA sequence read ATCGAT the mutated strand would read AACGTA. The highlighted letters are where a transversion mutation occurred. The pyrimidine thymine was replaced with a purine adenine. Another type of mutation is a transition mutation. This is very similar to a transversion mutation. In a transition mutation a purine is replaced with another purine or a pyrimidine is replaced with another pyrimidine. If your sequence read TAGCTATACG the mutated strand could read TAGTTATGCG. In your mutated strand the pyrimidine cytosine was replaced with another pyrimidine thymine. The purine adenine was replaced with another purine guanine. Insertions occur when extra base pairs are added into the DNA. So for example your DNA reads TAGTCG an insertion would be the highlighted area TAGGATTCG. A Deletion occurs when a section of the DNA is deleted. So in the same strand, TAGTCG, a deletion would result in the shortened strand of TAGG, where TC was deleted. Since DNA is read in codons an insertion or deletion can change the whole meaning of DNA’s message. So if your original strand read MAX WAS MAD the deletion of the first M would make the sentence not understandable: AXW ASM AD. There are other possible mutations but, all mutations have the ability to produce new phenotypes if they change the gene product. A mutation can have the three effects on an individual. In most cases it does not have an effect. Mutations create new alleles which is necessary for natural selection to occur.



No comments:

Post a Comment